Dror Emmanuel WARSCHAWSKI

Born May 25th, 1969

 

RESEARCH EXPERIENCE


Institut de Biologie Physico-Chimique, Paris (France)

Since 1998 Research associate at the Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS - UMR 7099. Advisor: Bruno Miroux.

Université du Québec à Montréal, Québec (Canada)

2015-2016 Associate professor. "Solid-state NMR of intact and living cells", Sabbatical, 1 year. Advisor: Isabelle Marcotte

2007 "Biophysical study of bicelles: NMR, atomic force microscopy, mass spectrometry, infra-red spectroscopy", Sabbatical, 1 year. Advisor: Isabelle Marcotte

Guelph University, Ontario (Canada)

1997 "Structural study of membrane peptides by solid-state NMR", Post-Doctorate, 3 months. Advisor: James H. Davis

Massachusetts Institute of Technology, Cambridge (USA)

95-97 "Lipids and membrane peptides under physiological conditions: structural and dynamical studies using solid-state NMR", Post-doctorate: 2 years. Advisor: Professor Robert G. Griffin

Université Paris Diderot, Paris (France)

92-95 "Biomembranes, model membranes: new approaches using magic-angle spinning solid-state NMR", Ph. D. in Molecular Biophysics , 3 years. Advisor: Professor Philippe F. Devaux

Guelph University, Ontario (Canada)

1992 "Lipid studies by solid-state NMR", 3 months. Advisor: Professor James H. Davis

Institut de Biologie Physico-Chimique, Paris (France)

1991 "Red blood cell study by EPR", 3 months. Advisor: Doctor Alain Zachowski

Institut Curie, Paris (France)

1990 "Study of an ion-plasma collision", 2 months

 

TEACHING EXPERIENCE


On the internet

I manage the mailing list of NMR laboratories around the world, as well as the two databases for protein structures determined by NMR:

http://www.drorlist.com/nmr.html
http://www.drorlist.com/rmnlist.html
http://www.drorlist.com/nmrlist.html
http://www.drorlist.com/nmr/MPNMR.html
http://www.drorlist.com/nmr/SPNMR.html


Agro Paris Tech

since 2009 Teaching biological NMR for the MSc "Protein engineering and industrial applications"

Canada

2016 Occasional teaching solid-state NMR at UQAM (Montréal) and Université Laval (Québec)

Université Paris Diderot

2006-2014 Teaching solid-state NMR for the MSc "Molecular and Cellular Biophysics"

2005-2014 Teaching biological NMR for the MSc "Structure, Proteomics and Functional Genetics"

2001-2014 Teaching practical NMR for the MSc in Biology

Université Paris-Sud 11

2006 Teaching biological solid-state NMR for the MSc "Structural and Functional Engineering of Biomolecules"

Université Paris Descartes

2004-2006 Member of the commission de spécialistes 31-32ème section

2001-2014 Teaching solid-state NMR for the MSc "Chemical and Biological Analyses"

Université Paris XIII Nord

2002-2009 Member of the commission de spécialistes 28ème section

Ecole Normale Supérieure, Paris

2006-2009 Teaching biological solid-state NMR for the MSc in Biology

2001 Teaching solid-state NMR for professors of "classes préparatoires aux grandes écoles"

Institut de Biologie Physico-Chimique, Paris

since 1998 Tutoring post-docs, PhD, MSc and BSc students

 

EDUCATION AND HONORS


Université Paris Diderot

2006 Habilitation to Direct Researches, Physics

92-95 Recipient of a fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche for a Ph. D. in Molecular Biophysics, Cum Magna Laude. Advisor: Philippe F. Devaux

91-92 DEA de Biophysique Moléculaire and Magistère de Physique, Honors AB

90-91 Maitrise de Physique (Masters in Physics), Honors AB

 

BIBLIOGRAPHY


M. Beaugrand, A. A. Arnold, A. Juneau, A. Balieiro Gambaro, D. E. Warschawski, P. T. F. Williamson and I. Marcotte "Magnetically-oriented bicelles with monoalkylphosphocholines – versatile membrane mimetics for Nuclear Magnetic Resonance applications". Langmuir 32:13244-13251 (2016)

X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "NMR can now scrutinize the membrane of a living cell" Atlas of Science, 16 June (2016)

X. L. Warnet, M. Laadhari, A. A. Arnold, I. Marcotte and D. E. Warschawski "A 2H magic-angle spinning solid-state NMR characterisation of lipid membranes in intact bacteria" Biochim. Biophys. Acta. 1858:146-152 (2016)

X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes" Biophys. J. 109:2461–2466 (2015)

G. Hattab, D. E. Warschawski, K. Moncoq and B. Miroux "Escherichia coli as host for membrane protein structure determination: a global analysis" Sci. Rep. 5:12097 (2015)

A. A. Arnold, B. Genard, F. Zito, R. Tremblay, D. E. Warschawski and I. Marcotte "Identification of lipid and saccharide constituents of whole microalgal cells by 13C solid-state NMR" Biochim. Biophys. Acta 1848:369-377 (2015)

G. Hattab, A. Y. T. Suisse, O. Ilioaia, M. Casiraghi, M. Dezi, X. L. Warnet, D. E. Warschawski, K. Moncoq, M. Zoonens and B. Miroux "Membrane Protein Production in Escherichia coli: Overview and Protocols" In Membrane Proteins Production for Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York, USA. Chapter 4, pp. 87-106 (2014)

L. J. Catoire, X. L. Warnet and D. E. Warschawski "Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR" In Membrane Proteins Production for Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York, USA. Chapter 12, pp. 315-345 (2014)

M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F. Williamson, and I. Marcotte "Lipid Concentration and Molar Ratio Boundaries for the Use of Isotropic Bicelles" Langmuir 30:6162-6170 (2014)


D. Warschawski "La fin du monde: la faute à qui?" Le Courrier (Genève), mardi 6 mai, page 4 (2014)

A. A. Arnold, B. Genard, A. L. D. A. G. Da Silva, R. Tremblay, D. E. Warschawski, F. Zito and I. Marcotte "Screening the Dynamics of Membrane Constituents in Intact Microalgal Cells by Solid-State NMR" Biophys. J. 106:508a (2014)

G. Hattab, K. Moncoq, D. Warschawski and B. Miroux "Escherichia Coli as Host for Membrane Protein Structure Determination: A Global Analysis" Biophys. J. 106:46a (2014)

A. Abdine, K.-H. Park and D. E. Warschawski "Cell-free membrane protein expression for solid-state NMR" Methods Mol. Biol. 831:85-109 (2012)

D. E. Warschawski, A. A. Arnold, M. Beaugrand, A. Gravel, E. Chartrand and I. Marcotte "Choosing membrane mimetics for NMR structural studies of transmembrane proteins" Biochim. Biophys. Acta 1808:1957-1974 (2011)

Cover of New Biotechnol. 28 (2011)A. Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free expression and labeling strategies for a new decade in solid-state NMR" New Biotechnol. 28:272-276 (2011)

A. Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier, C. Van Heijenoort and D. E. Warschawski "Structural study of the membrane protein MscL using cell-free expression and solid-state NMR" J. Magn. Reson. 204:155-159 (2010)

M. N. Triba, P. F. Devaux and D. E. Warschawski "Effects of lipid chain length and unsaturation on bicelles stability. A phosphorus NMR study" Biophys. J. 91:1357-1367 (2006)

M. N. Triba, M. Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski "Reconstitution and alignment by a magnetic field of a β-barrel membrane protein in bicelles" Eur. Biophys. J. 35:268-275 (2006)

D. E. Warschawski and P. F. Devaux "1H-13C polarization transfer in membranes: A tool for probing lipid dynamics and the effect of cholesterol" J. Magn. Reson. 177:166-171 (2005)

D. E. Warschawski and P. F. Devaux "Order parameters of unsaturated phospholipids in membranes and the effect of cholesterol: a 1H-13C solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996 (2005)

M. N. Triba, D. E. Warschawski and P. F. Devaux "Bicelles: disks or perforated lamellae?" Eur. Biophys. J. 34:710 (2005)

O. Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed supported lipid bilayers in nanotubes characterized by deuterium magic angle spinning NMR spectroscopy" Langmuir 21:3226-3228 (2005)

M.N. Triba, D. E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus NMR of lipid distribution in bicelles" Biophys. J. 88:1887–1901 (2005)

M. N. Triba, M. Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P. Gilard and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation of Surfactant Aqueous Suspensions" Journal of Colloid and Interface Science 274:341-345 (2004)

J.-L. Popot, E. A. Berry, D. Charvolin, C. Creuzenet, C. Ebel, D. M. Engelman, M. Flötenmeyer, F. Giusti, Y. Gohon, P. Hervé, Q. Hong, J. H. Lakey, K. Leonard, H. A. Shuman, P. Timmins, D. E. Warschawski, F. Zito, M. Zoonens, B. Pucci and C.Tribet "Amphipols: polymeric surfactants for membrane biology research" Cell. Mol. Life Sci. 60:1559-1574 (2003)

D. E. Warschawski and P. F. Devaux "Using DROSS to study the order of unsaturated lipid, sphingolipids and lipid / cholesterol interactions" Eur. Biophs. J. 32:288 (2003)

F. Ferrage, M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen "Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed Field Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545 (2003)

Correction: J. Am. Chem. Soc. 126:5654 (2004)

M. Traïkia, D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux "Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454 (2002)

N. Tsapis, R. Ober, A. Chaffotte, D. E. Warschawski, J. Everett, J. Kauffman, P. Kahn, M. Waks and W. Urbach "Modification of the elastic constants of a peptide-decorated lamellar phase", Langmuir 18:4384-4392 (2002)

D. E. Warschawski and P.F. Devaux "Polarisation transfer in lipid membranes" J. Magn. Resonance 145:367-372 (2000)

M. Traikia, D. E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux "Formation of unilameller vesicles by repetitive freeze-thaw cycles: characterization by electron microscopy and 31P-NMR" Eur. Biophs. J. 29:184-195 (2000)

D. E. Warschawski, M. Traikia, P. F. Devaux and G. Bodenhausen, "Solid-State NMR for the study of membrane systems: The use of anisotropic interactions" Biochimie 80:437-450 (1998)

D. E. Warschawski, J. D. Gross and R. G. Griffin, "Effects of membrane peptide dynamics on high-resolution magic-angle spinning NMR" Journal de Chimie Physique 95:460-466 (1998)

D. E. Warschawski, J. D. Gross and R. G. Griffin, "Dynamics of gramicidin A deduced from high-resolution magic-angle spinning NMR" Biophys. J. 72:A325 (1997)

J. D. Gross, D. E. Warschawski and R. G. Griffin, "Dipolar recoupling in MAS NMR: A probe for segmental order in lipid bilayers" J. Am. Chem. Soc. 119:796-802 (1997)

D. E. Warschawski, P. Fellmann and P. F. Devaux, "High resolution 31P-1H two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid mixtures spinning at the magic-angle" Eur. Biophys. J. 25:131-137 (1996)

J. D. Gross, P. R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F. Devaux and R. G. Griffin, "Multidimensional NMR in lipid systems. Coherence transfer through J couplings under MAS" J. Magn. Reson. B106:187-190 (1995)

D. E. Warschawski and P. F. Devaux, "Water suppression in 1H MASS NMR spectra of lipids and biological membranes" J. Magn. Reson. B106:76-79 (1995)

 

CONFERENCES


Organisation of the Workshop on NMR of Biological Solids in Paris (France, 2009, 2011, 2013)

 

REVIEWS


For: Biochemistry, Biophysical Journal, Colloid and Surfaces, CRAS Chimie, European Biophysics Journal, FEBS letters, Journal of  Membrane Biology, Magnetic Resonance in Chemistry, Magnetic Resonance in Medicine...

SKILLS


French, English, Hebrew, Spanish: Fluent

Computer languages used: BASIC, PASCAL, UNIX and VMS

Synthesis and purification of membrane peptides

 

INTERESTS


Creation of a music-band and public performances between 1984 and 1995

Traveling all over the world

Webmaster of several websites and mailing lists