Dror Emmanuel WARSCHAWSKI
Né le 25 Mai 1969
EXPERIENCE DE RECHERCHE
Sorbonne Univsersité, Paris
Depuis 2020 Chargé de recherche au Laboratoire des Biomolécules, CNRS - UMR 7203. Directeur: Olivier Lequin
Université du Québec à Montréal, Québec (Canada)
2015-2019 Professeur associé au Laboratoire de RMN des systèmes biologiques complexes. "RMN solide de cellules intactes et vivantes", Détachement, 4 ans. Directrice: Isabelle Marcotte
2007 Professeur invité au Laboratoire de RMN des systèmes biologiques complexes. "Etude biophysique de bicelles: RMN, microscopie à force atomique, spectrométrie de masse, spectroscopie infra-rouge", Mise à disposition, 1 an. Directrice: Isabelle Marcotte
1998-2015 Chargé de recherche au Laboratoire de Biologie Physico-Chimique des Protéines
Membranaires, CNRS - UMR 7099. Directeur: Bruno Miroux
Guelph University, Ontario (Canada)
1997 "Etude structurale de peptides membranaires par RMN solide", Post-Doctorat, 3 mois. Directeur: James H. Davis
Massachusetts Institute of Technology, Cambridge (USA)
95-97 "Lipides et peptides membranaires dans des conditions physiologiques: étude structurale et dynamique par RMN solide". Post-doctorat, 2 ans. Directeur: Robert G. Griffin
Université Paris Diderot, Paris (France)
92-95 "Membranes biologiques, membranes modèles: nouvelles approches utilisant la RMN solide en rotation à l'angle magique". Doctorat de Physique, spécialité Biophysique Moléculaire. 3 ans. Directeur: Philippe F. Devaux
Guelph University, Ontario (Canada)
1992 "Etude de lipides par RMN solide", 3 mois. Directeur: James H. Davis
1991 "Etude de globules rouges par RPE", 3 mois. Directeur: Alain Zachowski
Institut Curie, Paris
1990 "Etude d'une collision ion-plasma", 2 mois
EXPERIENCE D'ENSEIGNEMENT
Internet
Je gère la liste de diffusion des laboratoires de RMN de
par le monde:
2009-2015 et depuis 2020 Enseignement de la RMN
biologique au M1 "Bioingéniérie des protéines et applications
industrielles"
Canada
2016-2019 Enseignements occasionnels de la RMN des solides à l'Université de Montréal, l'UQAM (Montréal) et à l'Université Laval (Québec)
Université Paris Diderot
2006-2014 Enseignement de la RMN des solides au M2 recherche "Biophysique Moléculaire et Cellulaire"
2005-2014 Enseignement de la RMN biologique au M1
de Biologie
2001-2014 Enseignement pratique de la RMN au M2 recherche "Biophysique Moléculaire et Cellulaire"
2006 Enseignement de la RMN des solides biologiques au M2 recherche "Ingénierie structurale et fonctionnelle des biomolécules"
2004-2006 Membre de la commission de spécialistes de la 31-32ème section
2001-2014 Enseignement de la RMN solide au M2 professionnel "Analyses Chimiques et Biologiques"
2002-2009 Membre de la commission de spécialistes de la 28ème section
Ecole Normale Supérieure, Paris
2006-2009 Enseignement de la RMN solide biologique au M1 de Biologie
2001 Enseignement de la RMN solide pour les professeurs de classes préparatoires aux grandes écoles
depuis 1998 Encadrement de post-doctorants, d'étudiants en thèse, M2, M1, L3...
ETUDES
Université Paris Diderot
2006 Habilitation à Diriger des Recherches, UFR de Physique
92-95 Doctorat de Physique, spécialité Biophysique Moléculaire, Mention "Très honorable; Avec les félicitations du jury". Directeur: Philippe F. Devaux
91-92 DEA de Biophysique Moléculaire et Magistère de Physique, Mentions AB
89-91 Licence et Maîtrise de Physique, Mentions AB
Lycée Lakanal, Sceaux
88-89 Classe préparatoire aux grandes écoles d'ingénieur (Math. Spé. option P')
87-88 Classe préparatoire aux grandes écoles d'ingénieur (Math. Sup.)
1987 Baccalauréat C
BIBLIOGRAPHIE
Orcid : 0000-0002-4363-4965
Scopus : 6602213358
IDREF : 154796190
HAL : 887797
50) A. Poulhazan, A. A. Arnold, F. Mentink-Vigier, A. Muszyński, P. Azadi, A. Halim, S. Y. Vakhrushev, H. J. Joshi, T. Wang, D. E. Warschawski and I Marcotte "Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall" Nat. Commun. 15:986 (2024 ( https://hal.science/hal-04434789v1 ) https://doi.org/10.1038/s41467-024-45246-7
49)
K. Kumar, A. A. Arnold, R. Gauthier, M. Mamone, J.-F. Paquin, D. E.
Warschawski and I Marcotte "19F solid-state NMR approaches to probe
antimicrobial peptide interactions with membranes in whole cells"
Biochim. Biophys. Acta Biomembr. 1866:184269 (2024) ( https://hal.science/hal-04386127v1) https://doi.org/10.1016/j.bbamem.2023.184269
48) S. Beauclercq, O. Grenier, A. A. Arnold, D. E. Warschawski, G. H. Wikfors, B. Genard, R. Tremblay and I. Marcotte "Metabolomics and lipidomics reveal the effects of the toxic dinoflagellate Alexandrium catenella on immune cells of the blue mussel, Mytilus edulis" Harmful Algae 129:102529 (2023) (https://hal.science/hal-04268231v1) https://doi.org/10.1016/j.hal.2023.102529
47) K. Kumar, M. Sebastiao, A. A. Arnold, S. Bourgault, D. E. Warschawski and I. Marcotte "In situ solid-state NMR study of antimicrobial peptide interactions with erythrocyte membranes" Biophys. J. 121:1512-1524 (2022) (https://hal.science/hal-03656685v1) https://doi.org/10.1016/j.bpj.2022.03.009
46)
F. Laydevant, M. Mahabadi, P. Llido, J.-P. Bourgouin, L. Caron, A. A.
Arnold, I. Marcotte and D. E. Warschawski "Growth-phase dependence of
bacterial membrane lipid profile and labeling for in-cell solid-state
NMR applications" Biochim. Biophys. Acta 1864:183819
(2022) (https://hal.science/hal-03463942v1)
https://doi.org/10.1016/j.bbamem.2021.183819
45) A. Poulhazan, M. C. Dickwella Widanage, A. Muszyński, A. A. Arnold, D. E. Warschawski, P. Azadi, I. Marcotte and T. Wang "Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance" J. Am. Chem. Soc. 143:19374-19388 (2021) (https://hal.science/hal-03419267v2) https://doi.org/10.1021/jacs.1c07429
44) X.
Yan, K. Kumar, R. Miclette Lamarche, H. Youssef, G. S. Shaw, I.
Marcotte, C. E. DeWolf, D. E. Warschawski and E. Boisselier
"Interactions between the Cell Membrane Repair Protein S100A10 and
Phospholipid Monolayers and Bilayers" Langmuir 37:9652–9663
(2021) (https://hal.science/hal-03367662v1)
https://doi.org/10.1021/acs.langmuir.1c00342
43)
Z. Bouhlel, A. A. Arnold, J.-S. Deschênes, J.-L. Mouget, D. E.
Warschawski, R. Tremblay and I. Marcotte "Investigating the action of
the microalgal pigment marennine on Vibrio splendidus by in
vivo 2H and 31P solid-state NMR" Biochim. Biophys. Acta
Biomembr. 1863:183642 (2021)
(https://hal.science/hal-03242868v1) https://doi.org/10.1016/j.bbamem.2021.183642
42)
D. E.Warschawski, K. V. Lakshmi and I. Marcotte « Foreword to:
Biophysical studies of membrane systems and interactions - Commemorative
issue in honour of Professor Michèle Auger » Biochim. Biophys. Acta
Biomembr. 1863:183609 (2021) (https://hal.science/hal-03201135v1)
https://doi.org/10.1016/j.bbamem.2021.183609
41)
A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte
« Solid-state NMR study of microalgal membranes and cell
walls », in Solid-state NMR: Applications in
Biomembrane Structure (F. Separovic and M. A. Sani Eds.),
Institute of Physics Publishing, Bristol, UK. Chapter 4, pp. 1-23
(2020) (https://hal.science/hal-03089739v1)
https://doi.org/10.1088/978-0-7503-2532-5ch4
40) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte "Unambiguous Ex Situ and in Cell 2D 13C Solid-State NMR Characterization of Starch and Its Constituents" in In-Cell NMR Spectroscopy. Biomolecular Structure and Function (A. Shekhtman and D. S. Burz Eds.), MDPI Books, Basel, Switzerland. Chapter 8, pp. 122-138 (2020) (https://hal.science/hal-02569401v1) https://doi.org/10.3390/books978-3-03928-255-5
39)
A. E. Gravel, A. A. Arnold, M. Fillion, M. Auger, D. E. Warschawski and
I. Marcotte " Magnetically-orientable Tween-based Model Membranes for
NMR Studies of Proteins" Biochim. Biophys. Acta Biomembr. 1862:183379
(2020) (https://hal.science/hal-02861563v1)
https://doi.org/10.1016/j.bbamem.2020.183379
38) X.
Yan, F. Noël, I. Marcotte, C. E. DeWolf, D. E. Warschawski and E.
Boisselier "AHNAK C-Terminal Peptide Membrane Binding-Interactions
Between the Residues 5654-5673 of AHNAK and Phospholipid Monolayers and
Bilayers" Langmuir 36:362-369 (2020) (https://hal.science/hal-02567660v1)
https://doi.org/10.1021/acs.langmuir.9b02973
Z. Bouhlel, D. E. Warschawski, A. A. Arnold, K. Lemarchand, R. Tremblay, and I. Marcotte "Solid-State NMR of Intact Bacteria Reveals the Effect of Stress and Antimicrobial Agents" Biophys. J. 118:344a (2020)(https://hal.science/hal-03031123v1)
37)
Z. Bouhlel, A. A. Arnold, D. E. Warschawski, K. Lemarchand, R. Tremblay
and I. Marcotte "Labelling strategy and membrane characterization of
marine bacteria Vibrio splendidus by in vivo 2H NMR" Biochim.
Biophys. Acta 1861:871-878 (2019) (https://hal.science/hal-02567658v1)
https://doi.org/10.1016/j.bbamem.2019.01.018
36)
A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte
"Unambiguous Ex Situ and in Cell 2D 13C Solid-State NMR
Characterization of Starch and Its Constituents" Int. J. Mol. Sci.
19:3817 (2018) (https://hal.science/hal-02567657v1)
https://doi.org/10.3390/ijms19123817
35) E. Azar, D. Constantin
and D. E. Warschawski "The effect of gramicidin inclusions on the local
order of membrane components" Eur. Phys. J. E 41:44
(2018) (https://hal.science/hal-01744850v1)
https://doi.org/10.1140/epje/i2018-11644-5
34) D. E. Warschawski,
A. A. Arnold and I. Marcotte "A New Method of Assessing Lipid Mixtures
by 31P Magic-Angle Spinning NMR" Biophys. J. 114:1368–1376
(2018) (https://hal.science/hal-02567656v1)
https://doi.org/10.1016/j.bpj.2018.01.025
33)
A. A. Arnold, J.-P. Bourgouin,
B. Genard, D. E. Warschawski, R. Tremblay and I .Marcotte "Whole cell
solid-state NMR study of Chlamydomonas reinhardtii microalgae" J.
Biomol. NMR 70:123-131 (2018) (https://hal.science/hal-02567652v1)
https://doi.org/10.1007/s10858-018-0164-7
32) V. Booth, D. E.
Warschawski, N. P. Santisteban, M. Laadhari and I. Marcotte "Recent
progress on the application of 2H solid-state NMR to probe the
interaction of antimicrobial peptides with intact bacteria". Biochim. Biophys. Acta 1865:1500-1511
(2017) (https://hal.science/hal-02567650v1)
https://doi.org/10.1016/j.bbapap.2017.07.018
25) L. J. Catoire, X. L. Warnet and D. E. Warschawski "Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR" In Membrane Proteins Production for Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York, USA. Chapter 12, pp. 315-345 (2014) (https://hal.science/hal-02567627v1) https://doi.org/10.1007/978-1-4939-0662-8_12
24) M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F. Williamson, and I. Marcotte "Lipid Concentration and Molar Ratio Boundaries for the Use of Isotropic Bicelles" Langmuir 30:6162-6170 (2014) (https://hal.science/hal-01498056v1) https://doi.org/10.1021/la500435321) A. Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free expression and labeling strategies for a new decade in solid-state NMR" New Biotechnol. 28:272-276 (2011) (https://hal.science/hal-02567621v1) https://doi.org/10.1016/j.nbt.2010.07.014
20) A.
Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier,
C. Van Heijenoort and D. E. Warschawski "Structural study of the
membrane protein MscL using cell-free expression and solid-state NMR" J. Magn. Reson. 204:155-159
(2010) (https://hal.science/hal-02567620v1)
https://doi.org/10.1016/j.jmr.2010.02.003
19) M. N.
Triba, P. F. Devaux and D. E. Warschawski "Effects of lipid chain length
and unsaturation on bicelles stability. A phosphorus NMR study" Biophys.
J. 91:1357-1367 (2006) (https://hal.science/hal-02567618v1)
https://doi.org/10.1529/biophysj.106.085118
18) M. N.
Triba, M. Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski
"Reconstitution and alignment by a magnetic field of a β-barrel membrane
protein in bicelles" Eur. Biophys. J. 35:268-275
(2006) (https://hal.science/hal-02567617v1)
https://doi.org/10.1007/s00249-005-0014-x
17)
D. E. Warschawski and P. F. Devaux "1H-13C polarization transfer in
membranes: A tool for probing lipid dynamics and the effect of
cholesterol" J. Magn. Reson. 177:166-171 (2005) (https://hal.science/hal-02567616v1) https://doi.org/10.1016/j.jmr.2005.07.011
16)
D. E. Warschawski and P. F. Devaux "Order parameters of unsaturated
phospholipids in membranes and the effect of cholesterol: a 1H-13C
solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996
(2005) (https://hal.science/hal-02567615v1)
https://doi.org/10.1007/s00249-005-0482-z
15) O.
Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed
supported lipid bilayers in nanotubes characterized by deuterium magic
angle spinning NMR spectroscopy" Langmuir 21:3226-3228
(2005) (https://hal.science/hal-02567614v1)
https://doi.org/10.1021/la0469147
14) M.N. Triba, D. E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus NMR of lipid distribution in bicelles" Biophys. J. 88:1887–1901 (2005) (https://hal.science/hal-02567611v1) https://doi.org/10.1529/biophysj.104.055061
13) M. N.
Triba, M. Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P.
Gilard and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation
of Surfactant Aqueous Suspensions" Journal of Colloid and Interface
Science 274:341-345 (2004) (https://hal.science/hal-02567610v1)
https://doi.org/10.1016/j.jcis.2004.01.013
11) F.
Ferrage, M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen
"Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed
Field Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545
(2003) (https://hal.science/hal-02567606v1)
https://doi.org/10.1021/ja0211407
Correction: J. Am. Chem. Soc. 126:5654 (2004) (https://hal.science/hal-02567609v1)
10) M. Traïkia, D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux "Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454 (2002) (https://hal.science/hal-02568215v1) https://doi.org/10.1016/s0006-3495(02)73915-59) N.
Tsapis, R. Ober, A. Chaffotte, D. E. Warschawski, J. Everett, J.
Kauffman, P. Kahn, M. Waks and W. Urbach "Modification of the elastic
constants of a peptide-decorated lamellar phase", Langmuir 18:4384-4392
(2002) (https://hal.science/hal-02567603v1)
https://doi.org/10.1021/la015745c
8) D.
E. Warschawski and P.F. Devaux "Polarisation transfer in lipid
membranes" J. Magn. Resonance 145:367-372 (2000) (https://hal.science/hal-02567602v1) https://doi.org/10.1006/jmre.2000.2135
7) M.
Traikia, D. E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux
"Formation of unilameller vesicles by repetitive freeze-thaw cycles:
characterization by electron microscopy and 31P-NMR" Eur. Biophs. J.
29:184-195 (2000) (https://hal.science/hal-02567600v1)
https://doi.org/10.1007/s002490000077
6) D.
E. Warschawski, M. Traikia, P. F. Devaux and G. Bodenhausen,
"Solid-State NMR for the study of membrane systems: The use of
anisotropic interactions" Biochimie 80:437-450
(1998) (https://hal.science/hal-02567597v1)
https://doi.org/10.1016/s0300-9084(00)80011-5
5) D.
E. Warschawski, J. D. Gross and R. G. Griffin, "Effects of membrane
peptide dynamics on high-resolution magic-angle spinning NMR" Journal
de Chimie Physique 95:460-466 (1998) (https://hal.science/hal-02567599v1)
https://doi.org/10.1051/jcp:1998160
3) D. E. Warschawski, P. Fellmann and P. F. Devaux, "High resolution 31P-1H two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid mixtures spinning at the magic-angle" Eur. Biophys. J. 25:131-137 (1996) (https://hal.science/hal-02567595v1) https://doi.org/10.1007/s002490050024
2) J. D.
Gross, P. R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F.
Devaux and R. G. Griffin, "Multidimensional NMR in lipid systems.
Coherence transfer through J couplings under MAS" J. Magn.
Reson. B106:187-190 (1995) (https://hal.science/hal-02567594v1)
https://doi.org/10.1006/jmrb.1995.1031
1) D. E. Warschawski and P. F. Devaux, "Water suppression in 1H MASS NMR spectra of lipids and biological membranes" J. Magn. Reson. B106:76-79 (1995) (https://hal.science/hal-02567592v1) https://doi.org/10.1006/jmrb.1995.1012
CONFERENCES
RAPPORTS
Pour: BBA - Biomembranes, Biochemistry, Biophysical
Journal, Colloid and Surfaces, CRAS Chimie, European Biophysics Journal,
FEBS letters, Journal of Membrane Biology, Journal of Physical
Chemistry, Langmuir, Magnetic Resonance in Chemistry, Magnetic Resonance
in Medicine, Phytochemical Analysis, Scientific Reports...
AUTRES COMPETENCES
Informatique: BASIC, PASCAL, UNIX et VMS
Synthèse et purification de peptides membranaires en phase solide
AUTRES INTERETS
Nombreux voyages dans le monde entier
Responsable de plusieurs sites internet et de listes de diffusion